欧美在线视频免费观看_亚洲欧美日韩一区二区_国语成人_综合精品久久_不卡一二三区_亚洲国产精品自拍

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

FL62891細(xì)胞, 人肝細(xì)胞

簡要描述:FL62891細(xì)胞, 人肝細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-11005
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-05-25
  • 訪  問  量:2038

產(chǎn)品分類

Product Category

詳細(xì)介紹

FL62891細(xì)胞, 人肝細(xì)胞

ATCC® Number:CRL-11005™    Price:$353.00
Designations:FL 62891

Depositors:ImClone Systems Inc.

Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast

Source:Organ: liver
Cell Type: immortalized with SV40 large T antigen


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
FL62891細(xì)胞, 人肝細(xì)胞

Age:fetus

Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 32.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 32°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:48 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020



References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:[email protected]

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 国产一级黄色大片 | 五月婷婷综合激情网 | 国产人免费人成免费视频 | 日韩欧美一区二区三区免费观看 | 天天色天天射天天操 | 中文字幕精品一区 | 日本一区二区精品 | 午夜精品久久久久久久久 | 日韩在线国产精品 | 超碰最新网址 | 国产区久久 | 男人天堂99 | 久久黄色片 | 亚洲在线一区二区 | 午夜精品久久久久久久星辰影院 | 美女久久久久 | 天堂网中文在线 | 伊人亚洲 | 久久精品欧美一区二区三区不卡 | 精品九九九| 激情一区二区三区 | 97在线观看| 99久久久免费视频 | 欧美成人资源 | 国产欧美在线观看 | 国产三级毛片 | 狠狠爱亚洲| 国产精品久久免费视频 | 国产综合一区二区 | 狠狠艹| 日韩一区欧美一区 | 国产精品久久久久久久久免费 | 国产无区一区二区三麻豆 | ririsao久久精品一区 | 性免费网站 | 伊人一区二区三区 | 国产精品久久久久国产a级 日韩在线二区 | 精品久久中文字幕 | 国产一区亚洲二区三区 | 国产精品久久久久久久午夜 | 成人精品久久久 |